top of page
Yeast Genomic DNA Kit (GBYB100, GBYB300)

Yeast Genomic DNA Kit (GBYB100, GBYB300)

Yeast Genomic DNA Kit (GBYB100, GBYB300)

DNA Extraction/Genomic DNA Purification

The Presto™ Mini gDNA Yeast Kit was designed for rapid isolation of genomic DNA from cultured yeast and other fungus species. The yeast/fungus cells are directly added into beadbeating tubes containing ceramic beads and lysis buffer. Samples are efficiently lysed without the use of degestive enzymes (such as Lyticase or Zymolase) using a bead beating instrument or a standard vortex. The cell lysate is then mixed with Binding Buffer and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

 

DESCRIPTION

Specifications (Cat. # GBYB100, GBYB300)

  • Purify genomic DNA from (up to 2 x 108) yeast and other fungus species
  • Genomic DNA Yield: up to 10 µg of genomic DNA from 2 x 108 Saccharomyces cerevisiae
  • Format: beadbeating tubes and spin columns
  • Elution volume: 30-200 µl
  • Time: 40 minutes
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, restricion enzyme digestion and sequencing reactions

COMPONENTS

  • GT Buffer
  • PR Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer
  • RNase A (50 mg/m)
  • GD Columns
  • Beadbeating Tubes Type B
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Presto™ Mini gDNA Yeast DNA Kit is tested on a lot-to-lot basis by isolating genomic DNA from 2 x108 Saccharomyces cerevisiae cells. Following the purification process, greater than 8 μg of genomic DNA is obtained and the A260/A280 ratio is between 1.7-2.0. The purified genomic DNA is analyzed by electrophoresis.

Geneaid™ Yeast DNA Extraction Kit Funtional Test Data

Figure 1 .Genomic DNA was extracted using the Presto™ Mini gDNA Yeast DNA Extraction Kit. Saccharomyces cerevisiae (50 mg, 100 mg, 200 mg) was harvested by centrifugation at 5,000 x g for 10 minutes. 5 µl aliquots of purified genomic DNA from a 100 µl eluate were analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

 

Test

DNA Conc.

260/280

260/230

Yield

1

21.2 ng/µl

1.89

2.09

2.1 µg

2

22.2 ng/µl

1.90

2.05

2.2 µg

3

37.5 ng/µl

1.91

2.18

3.8 µg

4

40.7 ng/µl

1.90

2.05

4.1 µg

5

75.3 ng/µl

1.86

2.07

7.5 µg

6

84.9 ng/µl

1.87

2.11

8.5 µg

Genomic DNA Extraction Kits Specifications

 

SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM020
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)
50 µg50 µg50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 20 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KN/AProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue
  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109 Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl50-100 µl30-100 µl50-100 µl
Operation Time<20 minutes<20 minutes30 minutes<30 minutes<40 minutes<40 minutes<20 minutes<20 minutes

 

 

DOCUMENTS

    ₩0가격
    bottom of page