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Soil DNA Extraction Kit (SLD050, SLD100)

Soil DNA Extraction Kit (SLD050, SLD100)

Soil DNA Extraction Kit (SLD050, SLD100)

DNA Extraction/Genomic DNA Purification

The Presto™ Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized using SL1 Lysis Buffer combined with ceramic beads in Beadbeating Tubes. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire soil DNA extraction procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

 

DESCRIPTION

Specifications (Cat. # SLD050, SLD100)

  • Purify genomic DNA from soil within 40 minutes!
  • Sample: 250-500 mg of soil
  • High Yield: up to 5 µg
  • High Quality DNA: A260/A280 = 1.7-2.0
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

  • SL1 Buffer
  • SL2 Buffer
  • SL3 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Inhibitor Removal Columns
  • GD Columns
  • Beadbeating Tubes Type C
  • 2 ml Centrifuge Tubes
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Presto™ Soil DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 250 mg soil samples. Following the purification process, a yield of more than 2 μg of genomic DNA is obtained and the A260/A280 ratio is between 1.7-2.0. The purified genomic DNA is analyzed by electrophoresis.

 

Presto™ Soil DNA Extraction Kit Functional Test Data

Figure 1 .Total DNA was purified from 300 mg farm soil samples using three different soil DNA Extraction Kits. 10 µl aliquots of purified DNA from 100 µl eluates were analyzed on a 0.8% agarose gel.

M = Geneaid 1 Kb DNA Ladder, Lane 1-2: Geneaid Presto™ Soil DNA Extraction Kit, Lane 3-4: Brand M, Lane 5-6: Brand E

 

Product

Yield

260/280

260/230

Geneaid

2.2 µg

1.82

1.89

2.0 µg

1.83

1.91

Brand M

2.4 µg

1.79

1.20

2.5 µg

1.84

1.64

Brand E

1.5 µg

1.59

0.50

1.7 µg

1.55

0.55

Figure 2 .Total DNA was purified from 300 mg farm soil samples using the Geneaid Presto™ Soil DNA Extraction Kit and Brand M kit. Total DNA was eluted with 50 μl Elution Buffer. A Real-time PCR assay was performed with 1 μl of undiluted eluate (30 ng) as PCR template (3 replications), bacterial 16S rDNA universal primers, and Fast SYBR Green PCR Master Mix using the StepOnePlus™ Real-Time PCR system (Applied Biosystems). The average cycle threshold (Ct) from DNA extracted using the Geneaid kit is 13.15 compared to 13.66 from the Brand M kit. The lower Ct values indicate a higher number of target nucleic acid and less PCR inhibitors in the sample.

Figure 3 .Total DNA was purified from 300 mg farm soil samples using the Geneaid Presto™ Soil DNA Extraction Kit. 2 μl of undiluted eluates as template (3 replications) were analyzed by PCR using bacterial 16S rDNA universal primers. The results show that full length bacterial 16S rDNA fragments (1.5 kb) were successfully amplified.

Genomic DNA Extraction Kit Specifications

 

SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM020
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)
50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 20 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KN/AProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue
  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109 Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl30-200 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes

 

 

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