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RNA Cleanup Kit (PR050/PR100)

RNA Cleanup Kit (PR050/PR100)

RNA Cleanup Kit (PR050/PR100)

RNA Extraction/RNA Cleanup

The RNA Cleanup Kit uses a simple and efficient spin column procedure to purify total RNA stored in RNase-free water, elution buffer or TE Buffer following extraction using acid-guanidinium-phenol-chlorofom based methods such as TRIzol® Reagent and GENEzol™ Reagent. Contaminants such as RNases, DNA and residual phenol are effectively removed using a simple 4 step procedure. The high-quality, total RNA is eluted in RNase-free Water or TE (RNase-free) and is ready for use in a variety of sensitive downstream applications.

 

DESCRIPTION

Advantages (Cat. # PR050/PR100)

  • Purify up to 50 µg of total RNA in 10 minutes!
  • Compatibility: purify RNA stored in RNase-free water, elution buffer or TE buffer following RNA extraction using GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
  • Sample Volume: up to 100 μl of RNA products
  • Membrane: glass fiber membrane optimized for total RNA extraction
  • Spin Columns: individually packaged RNA spin columns, certified RNase and DNase-free
  • Elution Volume: 20-50 μl
  • Recovery: up to 80% of high-quality RNA (A260/A280 = 1.9-2.0)
  • Storage: room temperature (15-25ºC)

APPLICATIONS

RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protention Assay

COMPONENTS

  • RNA Pure Buffer
  • Wash Buffer
  • RNase-free Water
  • PR Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the RNA Cleanup Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Following RNA purifcation using the RNA Pure Kit, 10 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.

 

 

DOCUMENTS

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