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Presto™ Plasmid DNA Concentration Kit (PC0250/251, PC0500/501, PC1000/1001)

Presto™ Plasmid DNA Concentration Kit (PC0250/251, PC0500/501, PC1000/1001)

Presto™ Plasmid DNA Concentration Kit (PC0250/251, PC0500/501, PC1000/1001)

DNA Extraction/Plasmid DNA Purification

The Presto™ Plasmid DNA Concentration Kit offers a simplified replacement for time-consuming plasmid DNA precipitation procedures. The Presto™ Plasmid Concentrator will quickly and efficiently concentrate plasmid DNA in anion-exchange chromatographic DNA purification eluates collected from Geneaid Plasmid Midi Columns, Macherey-Nagel NucleoBond® AX, NucleoBond® Xtra Columns, QIAGEN® tip 500 Columns etc. Plasmid eluates are precipitated with isopropanol then passed through a Presto™ Plasmid Concentrator using a syringe. Once contaminants are removed using a proprietary Desalting Buffer, the concentrator is dried and plasmid DNA is eluted with a small volume (100-400 μl) of low salt Elution Buffer. The Presto™ Plasmid Concentrator eliminates DNA pellet loss and incomplete solubilization of precipitates to ensure highly concentrated, purified plasmid DNA. In only 10 minutes, the plasmid DNA can be used directly in a variety of sensitive downstream applications, such as transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.

 

DESCRIPTION

Specifications (Cat. # PC0250/251, PC0500/501, PC1000/1001)

  • Concentrate plasmid DNA in only 10 minutes compared to 60 minutes using traditional plasmid DNA precipitation!
  • Convenient: replaces traditional, time-consuming plasmid DNA precipitation procedures
  • Binding Capacity: 600 µg of plasmid DNA
  • Recovery: up to 90%
  • Concentration: up to 3.2 µg/µl
  • Plasmid Size: 1-20 kb
  • Elution Volume: 100-400 µl
  • Dead Volume: 25 µl
  • Operation Time: 10 minutes (manual), 20 minutes/6 preps (vacuum)
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun

COMPONENTS

  • Presto™ Plasmid Concentrator
  • Desalting Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • 20 ml syringe
  • 1 ml syringe

QUALITY CONTROL

The quality of the Presto™ Plasmid DNA Concentration Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.

Presto™ Plasmid DNA Concentrator DNA Recovery and Concentration

 

Input DNA

Elution Volume

 

100 µl

200 µl

400 µl

600 µl

100 µg

Recovery

65%

80%

90%

90%

Concentration

0.9 µg/µl

0.45 µg/µl

0.2 µg/µl

0.15 µg/µl

Eluate

75 µl

175 µl

375 µl

575 µl

300 µg

Recovery

55%

70%

80%

90%

Concentration

2.2 µg/µl

1.2 µg/µl

0.65 µg/µl

0.4 µg/µl

Eluate

75 µl

175 µl

375 µl

575 µl

600 µg

Recovery

40%

60%

75%

75%

Concentration

3.2 µg/µl

2.1 µg/µl

1.2 µg/µl

0.8 µg/µl

Eluate

75 µl

175 µl

375 µl

575 µl

Table 1.DNA Recovery is highly dependant on the volume of elution buffer used. 90% recovery can be acheived when using 600 μl of elution buffer. However, DNA concentration is decreased. When using small volumes of elution buffer, recovery is decreased but concentration is increased.

 

DOCUMENTS

 

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