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Magnetic Beads Virus DNA/RNA Extraction Kit II (MV048/MV096/MV480)

Magnetic Beads Virus DNA/RNA Extraction Kit II (MV048/MV096/MV480)

Magnetic Beads Virus DNA/RNA Extraction Kit II (MV048/MV096/MV480)

Magnetic Beads DNA/RNA Extraction/Magnetic Beads Virus DNA/RNA Kit

 

The Magnetic Beads Virus DNA/RNA Extraction Kit was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids, nasopharyngeal and oropharyngeal swabs in viral transport medium (VTM) and the supernatant of viral infected cell cultures. Viral DNA/RNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Viral DNA/RNA Kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA/RNA can be used in qPCR and qRT-PCR assays.

 

DESCRIPTION

Specifications (Cat. # MV048, MV096)

  • High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Magnetic Bead Concentration: 50 mg/ml
  • Magnetic Bead Size: ~ 5 µm
  • Sample: up to 200 µl of virus samples (plasma, serum, body fluid or nasopharyngeal and oropharyngeal swabs in viral transport medium (VTM) 
  • Manual or automated DNA/RNA isolation
  • Operation time: within 30 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC)

APPLICATIONS

RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS)

COMPONENTS

  • MV1 Buffer
  • W1 Buffer*
  • Wash Buffer*1
  • RNase-free Water
  • MV Magnetic Beads
  • Carrier RNA2
  • 96 Deep Well Plate
  • Adhesive Film

QUALITY CONTROL

The quality of the The Magnetic Beads Virus DNA/RNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.

Magnetic Beads Virus DNA/RNA Extraction Kit Functional Test Data

Figure 1 .Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Geneaid Magnetic Beads Virus DNA/RNA Extraction Kit (2 replications of each copy number). The purified RNA was eluted with 50 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 5 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV.

 

DOCUMENTS

 

 

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