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Magnetic Beads Genomic DNA Extraction Kit (Bacteria) ME048/ME096

Magnetic Beads Genomic DNA Extraction Kit (Bacteria) ME048/ME096

Magnetic Beads Genomic DNA Extraction Kit (Bacteria) ME048/ME096

Magnetic Beads DNA/RNA Extraction/Magnetic Beads gDNA kit Bacteria

The Magnetic Beads Genomic DNA Extraction Kit Bacteria was designed specifically for efficient genomic DNA purification from Gram (-) negative and Gram (+) positve bacteria. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications.

 

DOCUMENTS

 

DESCRIPTION

Advantages (Cat. # ME048, ME096)

  • High Yield: >26 µg of Genomic DNA
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Magnetic Bead Concentration: 50 mg/ml
  • Magnetic Bead Size: ~ 5 µm
  • Easily adapted to automated magnetic bead separation instruments and workstations
  • Sample: 2 x 109 of bacteria cells (Gram + and Gram -)
  • Manual or automated DNA isolation
  • Operation time: within 30 minutes (manual) 
  • Storage: dry at room temperature (15-25ºC), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

APPLICATIONS

qPCR, PCR, Real-time PCR, Automated Fluorescent DNA Sequencing, Southern Blotting, Next Generation Sequencing (NGS)

COMPONENTS

  • ME1 Buffer
  • ME2 Buffer
  • ME3 Buffer
  • ME4 Buffer
  • Lysozyme
  • Proteinase K
  • MW1 Buffer
  • MW2 Buffer
  • ME Magnetic Beads
  • Elution Buffer

QUALITY CONTROL

The quality of the The Magnetic Beads Genomic DNA Extraction Kit Bacteria is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating genomic DNA from 1 x 109 cells of Escherichia coli and from  1 x 109 cells of Bacillus subtilis. 5 μl of extracted DNA product from a 100 μl eluate is analyzed on a 0.8% agarose gel.

 

 

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