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ISOLATE DNA/RNA REAGENT KIT

ISOLATE DNA/RNA REAGENT KIT

Item Description

 

  • Sample Size: Scalable for blood, buffy coat, serum, plasma cultured cells and tissue
  • Operation time: up to 60 minutes
  • Can simultaneously extract DNA – RNA and Protein from the same sample.

IBI Isolate is a phenol, chloroform, and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA, and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells, and tissue.

For more details click on Protocol or Technical Information below.

 

Isolate Protocol

 

Technical Information

 

The extracted RNA can be used directly in a variety of downstream applications such as cDNA library construction, cloning, RT-PCR (Endpoint), real-time PCR, nuclease protection assays, and northern blotting.

Quality Control

IBI Isolate is tested on a lot-to-lot basis. RNA from a 1 ml human blood sample is extracted using IBI Isolate. 10 µl from a 50 µl eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.

How does the IBI Isolate DNA/RNA Reagent Sample Kit work?This kit uses a phenol-chloroform and guanidine isothiocyanate solution to simultaneously extract DNA, RNA and protein from a variety of samples. Once the sample is lysed, aqueous layers will form in the tube. The first, clear layer contains RNA, the second, white layer contains DNA & protein, the third, red layer contains organics, DNA and protein.

How do I just retrieve the protein from the White (inter-phase) and Red phase?Following DNA precipitation, add 100% ethanol into the inter-phase and organic phase, the proteins are in the phenol-ethanol supernatant. The proteins can be precipitated by adding isopropanol to the phenol-ethanol supernatant.

What kind of RNA yield can we expect from the Isolate Reagent Sample Kit?RNA was extracted from (1 x 106) SSN-1 cells (fish cell) by the IBI Isolate, the concentration of RNA is 500 ng/µl, and the total yield is found to be 25 µg RNA.

 

 

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