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HighPrep™ Total RNA Plus Kit

HighPrep™ Total RNA Plus Kit

HighPrep™ Total RNA Plus Kit

Magnetic bead-based kit designed to extract high-quality total RNA from mammalian tissues and cultured cells.

 

 

APPLICATIONS

 

Total RNA isolation for:

  • RT-qPCR
  • RNA sequencing
  • cDNA synthesis
  • Gene expression analysis

 

BENEFITS

 

  • High yield and quality total RNA purification
  • Adaptable to various automated liquid handling workstations
  • No toxic organic solvents

 

 

HighPrep™ Total RNA Plus Kit - Protocol (Size: 113 KB)

HighPrep™ Total RNA Plus Kit - SDS (Size: 1.1 MB)

MagBio Genomics Product List 2020 (Size: 286.2 KB)

HighPrep™ Total RNA Plus Datasheet (Size: 324.1 KB)

 

This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS

  • Prepare Sample and add Solution A
  • Add Pro K Solution
  • Add 100% Ethanol and Mag-D4 Particles and let the RNA bind to the beads
  • Wash using MRW1 Buffer, RWB2 Buffer and DNase mix.
  • Repeat wash steps
  • Elution done using RNA Elution Buffer 
  • Store the RNA at -20°C.

 

Kit Components

  • Solution A
  • RLB Buffer
  • DCE Buffer
  • MRW1 Buffer
  • RWB2 Buffer
  • Pro K Solution
  • DNase I
  • DNase I Digestion Buffer
  • RNA Elution Buffer
  • MAG-D4 Particles

 

Storage

 

MAG-D4 particles and Pro K Solution should be stored at 2-8°C. DNase A should be store at -20°C . All other components stored at 15-25°C.

Stability

14 months when kit components are stored as directed.

Disclaimers

 

For Research Use Only. Not for Use in Diagnostic Procedures.

 

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