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GPURE DNA ISOLATION KIT

GPURE DNA ISOLATION KIT

 

 

gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial, or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

 

Specifications

 

Sample:Adherent Cultured Animal cells (trypsinize cells prior to harvesting)
Suspension Cultured Animal cells
Format:Scalable DNA Precipitation Method
Yield:5 – 15 µg

 

gPure Cultured Cell Protocol

 

Technical Information

 

 

  • Item Description
  • Sample Size: scalable for cultured cells
  • Expectant Yield: high quality (A260/A280=1.8-2.0)
Cell Number0.5-1 x 1063-5 x 1063-5 x 107
Tube Size1.5 ml15 ml15 ml
Cell Lysis Buffer150 µl600 µl6 ml
RNAse A 10 mg/ml1 µl3 µl30 µl
Protein Removal Buffer50 µl200 µl2 ml
Isopropanol150 µl600 µl6 ml
70% Ethanol150 µl600 µl6 ml
DNA Hydration Buffer50 µl100 µl200 µl

 

 

 

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