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GENEzol™ 96 Well RNA Kit (96GZX04/96GZX10)

GENEzol™ 96 Well RNA Kit (96GZX04/96GZX10)

GENEzol™ 96 Well RNA Kit (96GZX04/96GZX10)

RNA Extraction/Tri RNA

The GENEzol™ 96 Well TriRNA Pure Kit is a phenol and guanidine isothiocyanate plus 96 well RNA binding plate system for high-throughput purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

DOCUMENTS

  • COMPLETE PROTOCOL

DESCRIPTION

Advantages (Cat. # 96GZX04, 96GZX10)

  • Purify 96 wells of total RNA within 1 hour
  • A cost effective phenol, guanidine isothiocyanate solution plus RNA binding plate system
  • No chlorophorm phase separation
  • No isopropanol RNA precipitation
  • No phenol carryover
  • Sample Volume: up to 150 μl of blood, buffy coat, serum, plasma, up to 2 x 106 cultured cells, 10-20 mg of tissue, up to 1 x 109 bacterial cells, 10-20 mg of plant tissue
  • High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  • Binding Capacity: 50 µg RNA per well
  • Binding Plates: glass fiber membrane optimized for total RNA extraction (certified RNase and DNase-free)
  • Elution Volume: 60-80 µl (dead volume: 20-25 µl)
  • Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, other components should be stored at room temperature (15-25ºC)

APPLICATIONS

cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

COMPONENTS

  • GENEzol™ Reagent
  • Pre-Wash Buffer
  • Wash Buffer
  • RNase-free Water
  • Presto™ RNA 96 Well Binding Plates
  • Microtubes (Racked)
  • Microtubes (8-strip)
  • Caps for Microtubes (8-strip)
  • 96 Deep Well Plates
  • 0.35 ml Collection Plates
  • Adhesive Film

QUALITY CONTROL

The GENEzol™ 96 Well TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.

 

DOCUMENTS

 

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