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Gel/PCR DNA Fragments Kit (DF100, DF300)

Gel/PCR DNA Fragments Kit (DF100, DF300)

Gel/PCR DNA Fragments Kit (DF100, DF300)

DNA Extraction/Gel Extraction

The Gel/PCR DNA Fragments Extraction Kit is a Gel Extraction Kit and PCR Cleanup Kit in 1 convenient system. This Gel Extraction Kit will recover or concentrate 70 bp-20 kb DNA fragments from agarose gel, PCR, or other enzymatic reactions. For processing 10-50 kb DNA fragments, the Large DNA Fragments Extraction Kit is available. For processing 40-200 bp DNA fragments, the Small DNA Fragments Extraction Kit is also available. The Gel/PCR DNA Fragments Extraction Kit uses chaotropic salt to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the gel/pcr spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions such as Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray and Transfection.

 

DESCRIPTION

Specifications (Cat. # DF100, DF300)

  • High Efficiency: up to 90% recovery from agarose gels and up to 95% recovery from PCR products or other enzymatic reactions
  • Convenient: gel extraction kit and PCR cleanup kit in one
  • Only 20 minute gel extraction or 10 minute PCR cleanup
  • Sample Size: up to 300 mg of agarose gel slice or up to 100 μl of PCR products
  • Broad Fragment Size Range: 70 bp–20 kb
  • Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup
  • Elution Volume: 20-50 µl
  • Storage: dry at room temperature (15-25ºC)

APPLICATIONS

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

  • DF Buffer
  • W1 Buffer
  • Wash Bufffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • DF Columns
  • 2 ml Collection Tubes

 

QUALITY CONTROL

The quality of the Gel/PCR DNA Fragments Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions and analyzed by electrophoresis.

 

Gel Extraction and PCR Cleanup Kits Specifications

 

SpecificationsGenepHlow™ Gel Extraction KitGenepHlow™ PCR Cleanup KitGenepHlow™ Gel/PCR KitGenepHlow™ DNA Cleanup Maxi KitPresto™ 96 Well Gel Extraction KitSmall DNA Fragments Extraction KitLarge DNA Fragments Extraction Kit
Cat. #DFG100
DFG300
DFC100
DFC300
DFH100
DFH300
DFM010
DFM025
96DFG04
96DFG10
DF101
DF301
DFL100
DFL300
FormatSpin ColumnSpin ColumnSpin ColumnMaxi Spin Column96 Well PlateSpin ColumnSilica Matrix
Binding Capacity10 µg10 µg10 µg200 µg10 µg10 µg5 µg
SampleAgarose Gel SlicePCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/Restriction Enzyme Digest ProductAgarose Gel SliceAgarose Gel Slice/PCR ProductAgarose Gel Slice/PCR Product
Fragment Size70 bp-20 kb70 bp-20 kb70 bp-20 kb100 bp-10 kb70 bp-20 kb40-200 bp100 bp-50 kb
pH IndicatorYesYesYesYesN/AN/AYes
RecoveryUp to 90%Up to 95%Up to 95%Up to 80%Up to 80%Up to 95%Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume20-50 µl20-50 µl20-50 µl500-1000 µl60-80 µl20-50 µl10-20 µl
Operation Time20 minutes10 minutes<20 minutes<25 minutes45 minutes<20 minutes<20 minutes

 

DOCUMENTS

 

 

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