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DNA Cleanup Maxi Kit (DFM010, DFM025)

DNA Cleanup Maxi Kit (DFM010, DFM025)

DNA Cleanup Maxi Kit (DFM010, DFM025)

DNA Extraction/Gel Extraction

GenepHlow™ DNA Cleanup Maxi Kits were designed to recover or concentrate DNA fragments from large volume agarose gel, restriction enzyme digestion or PCR reaction products. Gel/PCR Buffer (yellow color indicating optimal pH≤7.5) is premixed with a pH indicator to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0) which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes while DNA fragments are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.

 

DESCRIPTION

Specifications (Cat. # DFM010, DFM025)

  • High Efficiency: up to 80% recovery
  • Convenient: Gel/PCR binding buffer is premixed with a pH indicator to allow for easy determination of optimal pH
  • Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
  • Sample Size: up to 5 g of agarose gel slice or up to 2 ml of restriction enzyme digestion product or PCR product
  • Broad Fragment Size Range: 100 bp–10 kb
  • Operation Time: 24 minutes for enzymatic reaction cleanup and 36 minutes for gel extraction
  • Spin Columns: glass fiber membrane
  • Elution Volume: 500-1,000 µl (50 µl dead volume)
  • Binding Capacity: 200 µg
  • Storage: dry at room temperature (15-25ºC) for up to 18 months without showing any reduction in performance

APPLICATIONS

Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray, Transfection

COMPONENTS

  • Gel/PCR Buffer (premixed with a pH indicator)
  • 3M Sodium Acetate (pH5.0)
  • W1 Buffer
  • Wash Bufffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • DM Columns

QUALITY CONTROL

The quality of the GenepHlow™ DNA Cleanup Maxi Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA fragments are purified from either agarose gel, restriction enzyme digestion products, PCR products or other aqueous solutions analyzed by electrophoresis.

GenepHlow™ DNA Cleanup Maxi Kit Functional Test Data

Figure 1 .200 µg of pbluescript plasmid DNA was digested by Hind III endonuclease at 37ºC for 2 hours. Following digestion, the linear DNA fragment was purified using the GenepHlow™ DNA Cleanup Maxi Kit. The unpurified DNA fragment (lane 1) and 3 replications of 5 µl purified linear DNA fragments (lanes 2-4) were loaded on 0.8% agarose gel.

M = Geneaid 1 Kb DNA ladder

 

Sample

ng/µl

260/280

260/230

Elution

Yield (µg)

Recovery

2 - Purified DNA

155.4 ng/µl

1.87

2.17

1000 µl

155.4

77.7%

3 - Purified DNA

157.8 ng/µl

1.86

2.22

1000 µl

157.8

78.9%

4 - Purified DNA

152.6 ng/µl

1.87

2.24

1000 µl

152.6

76.3%

Figure 2.A pH indicator is premixed with the binding buffer to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow.

Gel Extraction and PCR Cleanup Kits Specifications

 

SpecificationsGenepHlow™ Gel Extraction KitGenepHlow™ PCR Cleanup KitGenepHlow™ Gel/PCR KitGenepHlow™ DNA Cleanup Maxi KitPresto™ 96 Well Gel Extraction KitSmall DNA Fragments Extraction KitLarge DNA Fragments Extraction Kit
Cat. #DFG100
DFG300
DFC100
DFC300
DFH100
DFH300
DFM010
DFM025
96DFG04
96DFG10
DF101
DF301
DFL100
DFL300
FormatSpin ColumnSpin ColumnSpin ColumnMaxi Spin Column96 Well PlateSpin ColumnSilica Matrix
Binding Capacity10 µg10 µg10 µg200 µg10 µg10 µg5 µg
SampleAgarose Gel SlicePCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/Restriction Enzyme Digest ProductAgarose Gel SliceAgarose Gel Slice/PCR ProductAgarose Gel Slice/PCR Product
Fragment Size70 bp-20 kb70 bp-20 kb70 bp-20 kb100 bp-10 kb70 bp-20 kb40-200 bp100 bp-50 kb
pH IndicatorYesYesYesYesN/AN/AYes
RecoveryUp to 90%Up to 95%Up to 95%Up to 80%Up to 80%Up to 95%Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume20-50 µl20-50 µl20-50 µl500-1000 µl60-80 µl20-50 µl10-20 µl
Operation Time20 minutes10 minutes<20 minutes<25 minutes45 minutes<20 minutes<20 minutes

 

DOCUMENTS

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