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Blood/Cell DNA Mini Kit (GB100, GB300)

Blood/Cell DNA Mini Kit (GB100, GB300)

Blood/Cell DNA Mini Kit (GB100, GB300)

DNA Extraction/Genomic DNA Purification

The Genomic DNA Mini Kit (Blood/Cultured Cell) is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood, buffy coat and cultured cell samples. This Genomic DNA Extraction Kit uses RBC Lysis Buffer and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or various other enzymatic reactions. For RNA purification from blood and cultured cell samples, the Total RNA Mini Kit (Blood/CC) is also available.



Specifications (Cat. # GB100, GB300)

  • Purify genomic DNA within 25 minutes!
  • High Yield, High Quality Genomic DNA: 4-10 μg of pure genomic DNA (A260/A280 = 1.8-2.0) from 300 µl of whole blood
  • Variety of Samples: up to 300 µl of whole blood, up to 200 µl of buffy coat, up to 1 x 107 cultured cells, up to 2 x 108 yeast and other fungus species
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)


PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR


  • RBC Lysis Buffer
  • GT Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • GD Columns
  • 2 ml Collection Tubes


The quality of the Genomic DNA Mini Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Genomic DNA from 300 μl of whole human blood is extracted using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA (4-10 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

gSYNC™ DNA Extraction Kit Functional Test Data

Figure 1 .Genomic DNA (20-30 kb) was extracted from 300 µl of whole blood using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA was eluted in 200 µl of Elution Buffer and 15 µl aliquots of the final sample were analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder


Genomic DNA Extraction Kits Specifications


SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)
50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 25 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KN/AProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue
  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109 Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl30-200 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes





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