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Bacteria Advanced DNA Kit (GBBA100, GBBA300)

Bacteria Advanced DNA Kit (GBBA100, GBBA300)

Bacteria Advanced DNA Kit (GBBA100, GBBA300)

DNA Extraction/Genomic DNA Purification

The Presto™ gDNA Bacteria Advanced Kit is designed for rapid isolation of genomic DNA from cultured Gram (+) positive and Gram (-) negative bacteria. After directly adding into pre-filled beadbeating tubes containing zirconia/ceramic beads and lysis buffer, bacterial cells are efficiently lysed using a standard vortex or bead beating instrument. Enzymatic digestion with Lysozyme and Proteinase K is not required. The cell lysate is then mixed with a binding buffer, followed by bind, wash and elute using a spin column designed specifically for bacteria DNA extraction. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 30 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

 

DESCRIPTION

Specifications (Cat. # GBBA100, GBBA300)

  • Purify genomic DNA within 30 minutes!
  • High Yield: up to 40 μg (1 x 109 Escherichia coli) and 15 µg (1 x 109 Bacillus subtilis)
  • High Purity gDNA: A260/A280 >1.9
  • DNA Size: 20-40 kb
  • Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109), 200 µl of blood and biological fluids such as plasma and urine
  • Convenient: includes bead beating tubes (pre-filled with zirconia/ceramic beads) for efficient cell lysis without the use of enzymes such as Lysozyme or Proteinase K
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
  • Elution volume: 30-200 µl
  • Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

COMPONENTS

  • GT Buffer
  • PR Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • RNase A (50 mg/ml)
  • Beadbeating Tubes Type A
  • GD Columns
  • 2 ml Collection Tubes

QUALITY CONTROL

The quality of the Presto™ gDNA Bacteria Advanced Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA from Escherichia coli cells (up to 1 x 109), (up to 40 µg with an A260/A280 ratio of >2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

 

Genomic DNA Extraction Kits Specifications

 

SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM020
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin Column
Binding Capacity50 µg50 µg50 µg50 µg (Mini)
500 µg (Maxi)
50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
 20 mg animal tissue200 µl buffy coat Maxi: 1 g plant tissue200 µl blood FTA® Cards 
 1x107 cultured cells1x107 cultured cells  1 ml urine, plasma 1 ml urine 
 hair2x108 yeast/fungus    1-100 µl saliva 
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme Proteinase KN/AProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
 10-40 µg from
20 mg tissue
  80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109 Bacillus subtilis   
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl30-200 µl30-100 µl50-100 µl
Operation Time<20 minutes<20 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes

 

DOCUMENTS

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